• 名称: OCI-LY3(人B细胞淋巴瘤)
  • 货号: CBP60265
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CBP60265
I. General information 
Synonyms: OCI-LY3
Background: established in 1983 from the bone marrow sample of a 52-year-old man with B-cell non-Hodgkin lymphoma (B-NHL, diffuse large B-cell lymphoma, DLBCL, stage 4B) at relapse; cells were described in the literature to be EBV-negative and lacking a classical t(8;14) translocation
Species: human (Homo sapiens)
Tissue: N/A 
Disease: B cell lymphoma
Gender: N/A 
Morphology: round cells growing singly or in clumps in suspension;
Growth Mode: N/A 
Doubling Time: ca. 24 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium: IMDM+ 20% FBS+0.05 mM  2-mercaptoethanol
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in PCR assay
Immunology: CD3 -, CD10 -, CD13 -, CD19 -, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa -, sm/cylambda +; in the referenced paper this cell line is described as CD19 +, cykappa +; image
Fingerprint: genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database
Species: N/A 
Cytogenetics: human flat-moded hypertriploid karyotype; 72-77<3n>XXYY, +1, +9, -10, +13, +14, -17, +19, +20, +22, der(1)t(1;17)(p13;q12)x2, der(4)t(4;18)(q31;q21)x2, del(6)(q13)x2, der(6)t(6;6)(p24;q12), der(7)t(6;7)(p24;p22), der(14)t(14;19)(q32;q13.3)x2, del(18)(q21), der(19)t(4;19)(q21;q13)t(4;18)(q31;q21)x2, der(19)t(14;19), dup(20)(q11q13)x2; sdl with der(6)t(6;12)(p21;q21), der(7)t(5;7)(?p15;p24) etc; resembles published karyotypes; carries cryptic t(14;19) with rearrangement of IGH and SPIB, and t(4;18) with copy number amplification of the BCL2 region
Virus   PCR: HBV -, HCV -, HIV-1 -, HIV-2 -, MLV -
Comments: For more information, please contact Cobioer (4008-750-250).

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