• 名称: HCC-15(人肺癌细胞(非小细胞))
  • 货号: CBP60096
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CBP60096
I. General information 
Synonyms: HCC-15
Background: established from the tumor of a 47-year-old man with non-small cell lung carcinoma (subtype squamous carcinoma); matched EBV+ B-lymphoblastoid cell line (B-LCL) is available (HCC-15BL)
Species: human (Homo sapiens)
Tissue: lung 
Disease: non-small cell lung carcinoma
Gender: N/A 
Morphology: adherent epitheloid cells growing as monolayer
Growth Mode: N/A 
Doubling Time: ca. 30-40 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium: RPMI-1640+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin +
Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile
Species: confirmed as human by cytogenetics
Cytogenetics: human hypodiploid karyotype with 16% polyploidy - 37(34-38)<2n>X, -X, -4, -13, -14, -17, -18, -19, -20, -22, +1-2mar, del(3)(q13), der(5;19)(q10;q10)add(19)(q13), add(9)(p2?), del(10)(p12-13), add(12)(q24.3), del(20)(?p12), add(22)(q1?) - sideline with add(8)(p12), del(7)(q21)
Virus   PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, MLV -, SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).

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