• 名称: HEK 293A(人胚肾细胞)
  • 货号: CBP60436
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CBP60436
I. General information 
Synonyms: HEK 293A
Species: Human
Tissue: embryonic kidney
Medium: DMEM+10%FBS+0.1 Mm NEAA
Disease: N/A
Cryopreservation medium: 90%FBS+10%DMSO
Comments: The 293A Cell Line is a subclone of the 293 Cell Line and has a relatively flat morphology. It facilitates the initial production, amplification and titering of replication-incompetent adenovirus. The cell line contains a stably integrated copy of the E1 gene that supplies the E1 proteins (E1a and E1b) required to generate recombinant adenovirus. The flat morphology of the cells makes the titering procedure easier.
Cell Line Overview Mutation Copy Number Variation
Gene Expression RNAi(siRNA/shRNA/sgRNA...) Compound Sensitivity Profile

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如下仅展示案例细胞A549数据:

 

Cell Line Overview: Download

Synonyms:

A 549; A549; NCI-A549; A549/ATCC; A549 ATCC; hA549

Background:

This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old Caucasian male. Cells were described to induce tumors in athmyic mice and to synthesize lecithin.

Species:

Homo sapiens, human

Tissue:

Lung

Disease:

Carcinoma

Gender:

Male, 58 years adult, Caucasian

Morphology:

Epithelial cells, growing adherently as monolayer

Growth Mode:

Adherent

Doubling Time:

24~40hrs

DNA Profile:

Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 11
D7S820: 8,11
THO1: 8,9.3
TPOX: 8,11

vWA: 14
Cobioer’s Cell Line Authentication Service

Culture Medium:

F-12K+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium:

90% Complete Medium+10%DMSO

Photo:

 

 

Mutation: Download
Gene Name(s) Gene Transcript AA Syntax CDS Syntax Mutation Type
AATK ENSG00000181409 ENST00000326724 p.E1219Q c.3655G>C Substitution - Missense
ABCA9 ENSG00000154258 ENST00000340001 p.M1I c.3G>T Substitution - Missense
ABCB1 ENSG00000085563 ENST00000265724 p.Y1267C c.3800A>G Substitution - Missense
ABI3BP ENSG00000154175 ENST00000471714 p.R104Q c.311G>A Substitution - Missense
ABI3BP ENSG00000154175 ENST00000284322 p.R104Q c.311G>A Substitution - Missense
ADH1A ENSG00000187758 ENST00000209668 p.I209V c.625A>G Substitution - Missense
AGTR1 ENSG00000144891 ENST00000349243 p.H132N c.394C>A Substitution - Missense

 

Copy Number Variation: Downlonad
Ensembl Gene Gene Name(s) Copy Number Signal Chromosome
ENSG00000237269 RBMY2TP 0 loss Y
ENSG00000227949 CYCSP46 0 loss Y
ENSG00000234803 FAM197Y2 0 loss Y
ENSG00000230066 FAM197Y3 0 loss Y
ENSG00000237802 FAM197Y6 0 loss Y
ENSG00000228383 FAM197Y7 0 loss Y
ENSG00000233699 TTTY18 0 loss Y
ENSG00000238073 RBMY2HP 0 loss Y

 

Gene Expression: Download
Gene Relative level of mRNA Gene Relative level of mRNA
NOC2L -1.66 SKI -0.75
KLHL17 0.15 MORN1 -0.43
PLEKHN1 0.51 RER1 -0.76
C1orf170 1.12 PEX10 -0.43
HES4 -0.22 PLCH2 0.34
ISG15 -3.09 PANK4 -1.64
AGRN 2.48 HES5 -1.87
RNF223 -1.74 TNFRSF14 -1.37

 

RNAi(siRNA/shRNA/sgRNA...):
Design according to the gene, please contact us 4008-750-250.

 

Compound Sensitivity Profile:Download
Drug Name  Targets  Z Score
Tanespimycin HSP90 -2.656303
Selumetinib MEK1, MEK2 -2.057352
NU7441 DNAPK -1.983103
KU-55933 ATM -1.962478
Refametinib MEK1, MEK2 -1.742238
TW 37 BCL2, BCL-XL, MCL1 -1.739538
AZD8055 MTORC1, MTORC2 -1.667451

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