• 名称: HCC-78(人肺癌细胞(非小细胞))
  • 货号: CBP60100
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CBP60100
I. General information 
Synonyms: HCC-78
Background: established from the pleural effusion of a 65-year-old man with adenocarcinoma of the lung, typed as non-small cell lung carcinoma; cells are described in the literature to carry a ROS1 rearrangement; matched EBV+ B-lymphoblastoid cell line (B-LCL) is available (HCC-78BL)
Species: human (Homo sapiens)
Tissue: lung
Disease: non-small cell lung carcinoma
Gender: N/A 
Morphology: large epitheloid cells growing in monolayers and aggregates; after trypsination cells are not always dispersed as single cells; image; image
Growth Mode: N/A 
Doubling Time: ca. 60-70 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium: RPMI-1640+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 (+), cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin +
Fingerprint: fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile
Species: confirmed as human by PCR speciation and cytogenetics
Cytogenetics: human flat-moded complex hypotetraploid karyotype with 6% polyploidy - 80-91<4n>XYY/XXYY, add(X)(q25)x2, add(1)(?p11), add(4)(p15), add(4)(q31), add(16)(q23), der(16)t(1;16)(p12;q23)x2, dup(16)(q11q13)add(16)(q13), add(19)(q13)x2, inc.
Virus   PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).

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