• 名称: WSU DLCL2(人淋巴瘤细胞)
  • 货号: CBP60273
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CBP60273
I. General information 
Synonyms: WSU DLCL2
Background: derived from the pleural effusion of a 41-year-old Caucasian man with B-cell non-Hodgkin lymphoma (B-NHL, diffuse large cell lymphoma non-cleaved type, intermediate grade - transformed from follicular small cleaved cell, low grade) at the status of relapse/progressive disease in 1990; cell line carries EZH2 Y641F mutation
Species: human (Homo sapiens)
Tissue: N/A 
Disease: B cell lymphoma
Gender: N/A 
Morphology: round and oval cells growing singly or in small clumps in suspension; 
Growth Mode: N/A 
Doubling Time: ca. 50-70 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium: RPMI-1640+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: contamination was eliminated with Plasmocin, then negative in microbiological culture, PCR assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 -, CD 138 -, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa -, sm/cylambda +; image
Fingerprint: fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile
Species: confirmed as human by cytogenetics and species PCR
Cytogenetics: human hyperdiploid karyotype - 47(43-48)<2n>XY, +7, +8, t(1;2)(p36;q37), del(3)(q25), t(4;14)(q27;q31), der(7)t(3;7)(q25;p13), i(7p), add(8)(q24)x2, del(13)(?q21?q22), t(14;18)(q32;q21), del(15)(q25q27) - carries t(14;18) effecting BCL2-IGH fusion - matches published karyotype
Virus   PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).

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