• 名称: mIMCD-3(小鼠肾髓质细胞)
  • 货号: CBP60600
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CBP60600
I. General information 
Synonyms: mIMCD-3
Background:  mIMCD-3 is an inner medullary collecting duct (IMCD) cell line derived in 1991 by Michael Rauchman from a mouse transgenic for the early region of SV40 [Tg(SV40E)bri/7]. A tubule from the terminal one-third of the IMCD was microdissected and placed in culture. Confluent cells were subcultured and cloned using cloning cylinders.
Species: Mus musculus, transgenic, mouse, transgenic
Tissue: kidney, medulla/collecting duct
Gender: adult
Morphology: epithelial
Growth Mode: adherent
DNA Profile: Cobioer’s Cell Line Authentication Service
Culture Medium: DMEM/F12+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Comments: This is a polarized epithelial cell line which retains many differentiated characteristics of the terminal IMCD including inhibition of apical to basal sodium flux by amiloride and by atrial natriuretic peptide (ANP). he cells possess an amiloride sensitive sodium channel as determined by Western blot analysis, and accumulate the major organic osmolytes (inositol, sorbitol, betaine and glycerophosphorylcholine) in response to hypertonic stress.
The cells secrete endothelin and form tubules and tight junctions.mIMCD-3 cells are responsive to Hepatocyte Growth Factor (HGF), and are readily adaptable to growth in hypertonic medium supplemented with NaCl and urea up to 910 mosmol/kg H20.  These extreme osmotic conditions exist in the renal medulla in vivo, but are known to be lethal to most other cells.
For more information, please contact Cobioer (4008-750-250).

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