• 名称: U251(人脑胶质瘤细胞)
  • 货号: CBP60300
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CBP60300
I. General information 
Synonyms: U251
Background: Derived from a malignant glioblastoma tumour by explant technique. U-251 was formerly distributed as U-373 MG (ECACC catalogue number 89081403) until short tandem repeat (STR)-PCR profiling confirmed identity with U-251. A new deposit of U-373 MG known as U-373 MG (Uppsala) is now available (ECACC catalogue number 08061901).
Background to the identity query for the cell line U-373 MG:  The American Type Culture Collection (ATCC) reported that their stock of U-373 MG had been shown to have differing genetic properties to stock from the originator’s laboratory, and to share similarities with another glioblastoma cell line, U-251.  In light of this, ECACC undertook an investigation into the authenticity of its own stock of the U-373 MG cell line.  ECACC found similar results to the ATCC i.e. the stock held as U-373 MG was found to be identical by STR-PCR profiling to U-251.  The U-373 MG cell line listed under catalogue number 89081403 has been re-named as ‘U-251 MG (formerly known as U-373 MG)’ and has the new ECACC catalogue number 09063001.
Species: human (Homo sapiens)
Tissue: Brain
Disease: Human glioblastoma astrocytoma
Gender: N/A
Morphology: Pleomorphic/astrocytoid
Growth Mode: Adherent
Doubling Time: N/A
DNA Profile: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 10,11
D16S539: 12
D5S818: 11,12
D7S820: 10,12
THO1: 9.3
TPOX: 8
vWA: 16,18
Cobioer’s Cell Line Authentication Service
Culture Medium: MEM+1%NEAA+1mM NaP+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Karyotype: 2n = 46
Subculture Routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Comments: For more information, please contact Cobioer (4008-750-250).
Cell Line Overview Mutation Copy Number Variation
Gene Expression RNAi(siRNA/shRNA/sgRNA...) Compound Sensitivity Profile

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如下仅展示案例细胞A549数据:

 

Cell Line Overview: Download

Synonyms:

A 549; A549; NCI-A549; A549/ATCC; A549 ATCC; hA549

Background:

This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old Caucasian male. Cells were described to induce tumors in athmyic mice and to synthesize lecithin.

Species:

Homo sapiens, human

Tissue:

Lung

Disease:

Carcinoma

Gender:

Male, 58 years adult, Caucasian

Morphology:

Epithelial cells, growing adherently as monolayer

Growth Mode:

Adherent

Doubling Time:

24~40hrs

DNA Profile:

Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 11
D7S820: 8,11
THO1: 8,9.3
TPOX: 8,11

vWA: 14
Cobioer’s Cell Line Authentication Service

Culture Medium:

F-12K+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium:

90% Complete Medium+10%DMSO

Photo:

 

 

Mutation: Download
Gene Name(s) Gene Transcript AA Syntax CDS Syntax Mutation Type
AATK ENSG00000181409 ENST00000326724 p.E1219Q c.3655G>C Substitution - Missense
ABCA9 ENSG00000154258 ENST00000340001 p.M1I c.3G>T Substitution - Missense
ABCB1 ENSG00000085563 ENST00000265724 p.Y1267C c.3800A>G Substitution - Missense
ABI3BP ENSG00000154175 ENST00000471714 p.R104Q c.311G>A Substitution - Missense
ABI3BP ENSG00000154175 ENST00000284322 p.R104Q c.311G>A Substitution - Missense
ADH1A ENSG00000187758 ENST00000209668 p.I209V c.625A>G Substitution - Missense
AGTR1 ENSG00000144891 ENST00000349243 p.H132N c.394C>A Substitution - Missense

 

Copy Number Variation: Downlonad
Ensembl Gene Gene Name(s) Copy Number Signal Chromosome
ENSG00000237269 RBMY2TP 0 loss Y
ENSG00000227949 CYCSP46 0 loss Y
ENSG00000234803 FAM197Y2 0 loss Y
ENSG00000230066 FAM197Y3 0 loss Y
ENSG00000237802 FAM197Y6 0 loss Y
ENSG00000228383 FAM197Y7 0 loss Y
ENSG00000233699 TTTY18 0 loss Y
ENSG00000238073 RBMY2HP 0 loss Y

 

Gene Expression: Download
Gene Relative level of mRNA Gene Relative level of mRNA
NOC2L -1.66 SKI -0.75
KLHL17 0.15 MORN1 -0.43
PLEKHN1 0.51 RER1 -0.76
C1orf170 1.12 PEX10 -0.43
HES4 -0.22 PLCH2 0.34
ISG15 -3.09 PANK4 -1.64
AGRN 2.48 HES5 -1.87
RNF223 -1.74 TNFRSF14 -1.37

 

RNAi(siRNA/shRNA/sgRNA...):
Design according to the gene, please contact us 4008-750-250.

 

Compound Sensitivity Profile:Download
Drug Name  Targets  Z Score
Tanespimycin HSP90 -2.656303
Selumetinib MEK1, MEK2 -2.057352
NU7441 DNAPK -1.983103
KU-55933 ATM -1.962478
Refametinib MEK1, MEK2 -1.742238
TW 37 BCL2, BCL-XL, MCL1 -1.739538
AZD8055 MTORC1, MTORC2 -1.667451

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