• 名称: U251(人脑胶质瘤细胞)
  • 货号: CBP60300
  • 浏览次数: 1957
  • 已销售数量:0件
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CBP60300
I. General information 
Synonyms: U251
Background: Derived from a malignant glioblastoma tumour by explant technique. U-251 was formerly distributed as U-373 MG (ECACC catalogue number 89081403) until short tandem repeat (STR)-PCR profiling confirmed identity with U-251. A new deposit of U-373 MG known as U-373 MG (Uppsala) is now available (ECACC catalogue number 08061901).
Background to the identity query for the cell line U-373 MG:  The American Type Culture Collection (ATCC) reported that their stock of U-373 MG had been shown to have differing genetic properties to stock from the originator’s laboratory, and to share similarities with another glioblastoma cell line, U-251.  In light of this, ECACC undertook an investigation into the authenticity of its own stock of the U-373 MG cell line.  ECACC found similar results to the ATCC i.e. the stock held as U-373 MG was found to be identical by STR-PCR profiling to U-251.  The U-373 MG cell line listed under catalogue number 89081403 has been re-named as ‘U-251 MG (formerly known as U-373 MG)’ and has the new ECACC catalogue number 09063001.
Species: human (Homo sapiens)
Tissue: Brain
Disease: Human glioblastoma astrocytoma
Gender: N/A
Morphology: Pleomorphic/astrocytoid
Growth Mode: Adherent
Doubling Time: N/A
DNA Profile: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 10,11
D16S539: 12
D5S818: 11,12
D7S820: 10,12
THO1: 9.3
TPOX: 8
vWA: 16,18
Cobioer’s Cell Line Authentication Service
Culture Medium: MEM + 10% FBS + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP)
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Karyotype: 2n = 46
Subculture Routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Comments: For more information, please contact Cobioer (4008-750-250).

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