| CBP60302 |
| I. General information |
| Synonyms: |
LN229 |
| Background: |
The LN-229 cell line was established in 1979 from cells taken from a patient with right frontal parieto-occipital glioblastoma [PubMed. 10416987]. |
| Species: |
Homo sapiens, human |
| Tissue: |
brain/right frontal parieto-occipital cortex |
| Disease: |
glioblastoma |
| Gender: |
female，60 years，White |
| Morphology: |
epithelial |
| Growth Mode: |
adherent |
| DNA Profile: |
Amelogenin: X
CSF1PO: 12
D13S317: 10,11
D16S539: 12
D5S818: 11,12
D7S820: 8,11
THO1: 9.3
TPOX: 8
vWA: 16,19
Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
DMEM+5%FBS
We strongly suggest to purchase the complete medium from Cobioer. |
| Cryopreservation medium: |
90%FBS+10%DMSO |
| Oncogene: |
p53 + (mutated, CCT (Pro) --> CTT (Leu) mutation at codon 98), PTEN + (wild type), p16 - (deleted), p14ARF - (deleted) |
| Tumor Formation: |
Yes, forms tumors in nude mice |
| Comments: |
The cells exhibit mutated p53 (TP53) and possible homozygous deletions in the p16 and p14ARF tumor suppressor genes. They have a wild-type PTEN gene.
Stimulation of the cells with Fas ligand lead to apoptotic cell death within 16 hours. The cells were also killed by puromycin in a dose dependent manner.
Bcl-2 protects these cells from Fas ligand-induced cell death but was shown to have only a small protective effect on puromycin-induced apoptosis.
For more information, please contact Cobioer (4008-750-250). |
