• 名称: NCI-H358(人非小细胞肺癌细胞)
  • 货号: CBP60136
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CBP60136
I. General information 
Synonyms: NCI-H358
Background: This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates in 1981 from tumor tissue obtained from a patient prior to initiation of chemotherapy.
Species: Homo sapiens, human
Tissue: lung/bronchiole; derived from metastatic site: alveolus
Disease: bronchioalveolar carcinoma; non-small cell lung cancer
Gender: male
Morphology: epithelial
Growth Mode: adherent
Doubling Time: 38 hrs
DNA Profile: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,12
D16S539: 12,13
D5S818: 10,12
D7S820: 10,11
THO1: 6
TPOX: 8,9
vWA: 17
Cobioer’s Cell Line Authentication Service
Culture Medium: RPMI-1640+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Genes Expressed: lung surfactant associated protein A (SP-A),The cells expressed protein and RNA of SP-A, the major lung surfactant associated protein.
Cellular Products: lung surfactant associated protein A (SP-A)
Tumor Formation: Yes, the cells produce tumors in athymic nude mice
Comments: Ultrastructural studies demonstrated the presence of cytoplasmic structures characteristic of Clara cells.The cells expressed protein and RNA of SP-A, the major lung surfactant associated protein.
SP-B and SP-C RNA was not expressed.They have a reported colony forming efficiency of 0.83% in soft agarose.
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