• 名称: NCI-H520(人肺癌鳞癌细胞)
  • 货号: CBP60139
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CBP60139
I. General information 
Synonyms: NCI-H520
Background: The NCI-H520 cell line was derived by A.F. Gazdar and associates in 1982 from a sample of a lung mass taken from a patient with squamous cell carcimoma of the lung.
Species: Homo sapiens, human
Tissue: lung
Disease: squamous cell carcinoma
Gender: male
Morphology: epithelial
Growth Mode: adherent
Doubling Time: 61 hrs in medium with serum; 25 to 32 hrs in serum-free medium
DNA Profile: Amelogenin: X
CSF1PO: 10x
D13S317: 10,11
D16S539: 8,13
D5S818: 12,13
D7S820: 8,12
THO1: 10
TPOX: 8
vWA: 18,19
Cobioer’s Cell Line Authentication Service
Culture Medium: RPMI-1640+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Tumor Formation: Yes, in nude mice inoculated subcutaneously with 10(7) cells
(Tumors developed within 21 days at 100% frequency (5/5).)
Comments: The cells express greatly reduced levels of p53 mRNA relative to normal lung tissue, but exhibit no gross structural DNA abnormalities.
The cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein.
The line can be cloned in soft agar (with or without serum).
For more information, please contact Cobioer (4008-750-250).

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