• 名称: M059K(人脑恶性胶质母细胞瘤)
  • 货号: CBP60597
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CBP60597
I. General information 
Synonyms: M059K
Background: M059K cells were isolated from a tumor specimen from a 33 year-old male patient with untreated glioblastoma. These cells were isolated concurrently from the same tumor specimen as M059J (see ATCC CRL-2366). ­
Species: Homo sapiens, human
Tissue: brain
Disease: malignant glioblastoma; glioma
Gender: male,33 years
Morphology: fibroblast
Growth Mode: adherent
DNA Profile: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 14
D16S539: 10,12
D5S818: 11,12
D7S820: 10
THO1: 9.3
TPOX: 8
vWA: 17
Cobioer’s Cell Line Authentication Service
Culture Medium: Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP)
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Tumor Formation: Yes, forms tumors in SCID mice
Comments: M059K cells express normal levels of DNA-dependent protein kinase while M059J cells lack DNA-dependent protein kinase activity. M059K cells are approximately 30-fold less sensitive to ionizing radiation than M059J cells. M059K cells are less sensitive than M059J cells to the cytotoxic effects of bleomycin, N, N-bis(2-chloroethyl)-N-nitrosourea and nitrogen mustard. M059K cells are DNA double strand break repair proficient. Both cell lines are negative for glial fibrillary acidic acid (GFAP). Together these cell lines provide useful model systems in which to study the role of DNA protein kinase in cellular and molecular processes involving DNA damage recognition and repair.
For more information, please contact Cobioer (4008-750-250).

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