• 名称: I 2.1(人急性淋巴细胞白血病细胞)
  • 货号: CBP60789
  • 浏览次数: 158
  • 已销售数量:0件
  • 评论信息
  • 购买数量:  购买数量不能低于最少订购数量!

购买 询价 收藏 QQ交流 好友推荐

CBP60789
I. General information 
Synonyms: I 2.1
Background: The I 2.1 cell line is a FADD mutant of the wild-type Jurkat cell line A3 (see ATCC -CRL-2570). Wild-type A3 cells were made neomycin resistant and treated with three cycles of exposure to the frameshifting mutagen ICR-191 to isolate clones harboring recessive mutations that were resistant to killing by Fas antibody. Ref
ICR-191 treated clones were serially diluted in 96-well plates in the presence of Fas Antibody for 3 to 5 weeks. Ref Two of these ICR-191 treated clones have been deposited at the ATCC . They are I 9.2 (ATCC CRL-2571), a clone with a mutation in the cysteine protease caspase-8/FLICE and I 2.1 (ATCC CRL-2572), a clone with a mutation in the adaptor FADD. 
Species: Homo sapiens, human
Disease: acute T cell leukemia
Gender: male
Morphology: lymphoblast
Growth Mode: suspension
DNA Profile: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,11
D16S539: 11
D5S818: 9
D7S820: 8,10
THO1: 6,9.3
TPOX: 8,10
vWA: 17,18
Cobioer’s Cell Line Authentication Service
Culture Medium: RPMI-1640+15%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Comments: Unlike the parental line A3 that does express FADD, the I 2.1 cell line does not express FADD protein by immunoblot analysis and is completely resistant to Fas-induced death. Complementation of the I 2.1 cell lines with wild-type FADD restores Fas-mediated apoptosis. Fas activation of caspase-2, caspase-3, caspase-7, and caspase-8 is completely defective in the FADD mutant cell line I 2.1. Ref
This cell line can be used to study the role of FADD in apoptotic signaling pathways in the absence of overxpression.
For more information, please contact Cobioer (4008-750-250).

关键词: