• 名称: M-07e(人巨细胞白血病细胞)
  • 货号: CBP60791
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CBP60791
I. General information 
Synonyms: M-07e
Background: established from the peripheral blood of a 6-month-old girl with acute megakaryoblastic leukemia (AML M7) at diagnosis in 1987; subline of the growth factor-independent cell line M-07; M-07e cells respond proliferatively to GM-CSF, IFN-alpha, IFN-ß, IFN-gamma, IL-2, IL-3, IL-4, IL-6, IL-15, NGF, SCF, TNF-alpha, TPO; we have noted that the cell line can become very quickly cytokine-independent (within 3-4 weeks), presumably due to outgrowth of independent cells
Species: Homo sapiens, human
Tissue: operipheral blod
Disease: acute megakaryoblastic leukemia
Gender: famale,6-month-old  
Morphology: acute megakaryoblastic leukemia
Growth Mode: single round cells in suspension, a few cells are lightly adheren
Doubling Time: 40 hours
Culture Medium: RPMI-1640 + 20% FBS + GM-CSF (10 ng/ml) or 10% vol conditioned medium of cell line 5637
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Comments: Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays                                                                                                                        Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Immunology: CD3 -, CD13 +, CD14 -, CD19 -, CD33 +, HLA-DR                         Cytogenetics: human near-diploid karyotype - 46(45-46)<2n>XX, t(11;21)(p11;p13), add(13)(p13), add(22)(p13)
For more information, please contact Cobioer (4008-750-250).

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