• 名称: NCI-H209(人小细胞肺癌细胞)
  • 货号: CBP60793
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CBP60793 
I. General information 
Synonyms: NCI-H209
Background: The NCI-H209 cell line was derived by A.F. Gazdar and associates in 1979 from the bone marrow of a patient with small cell cancer of the lung.
Species: Homo sapiens, human
Tissue: lung; derived from metastatic site: bone marrow
Disease: carcinoma; small cell lung cancer (SCLC)
Gender: male,Caucasian
Morphology: epithelial
Growth Mode: aggregates in suspension
DNA Profile: Amelogenin: X,Y
CSF1PO: 11
D13S317: 11
D16S539: 9,12
D5S818: 12
D7S820: 9
THO1: 7,9
TPOX: 8
vWA: 18,19
Cobioer’s Cell Line Authentication Service
Culture Medium: RPMI-1640+20%FBS+0.05 mM 2-mercaptoethanol
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Oncogene: pRB + (abnormal, RB1)
Tumor Formation: Yes, forms transplantable tumors with typical SCLC histology in nude mice
Comments: C-myc DNA sequences are not amplified.
No gross structural DNA abnormalities were detected.
This is a cell line that grows as large aggregates in suspension. Only the aggregates are viable, but no meaningful viability percentage can be measured. The medium will normally contain large amounts of cell debris.
The cells express an aberrant form of RB1 that is not phosphorylated, apparently due to a single point mutation at codon 706 (Cys -> Phe).The line is a classic SCLC cell line which expresses elevated levels of four biochemical markers (neuron specific enolase, brain isoenzyme of creatine kinase, L-DOPA decarboxylase and bombesin-like immunoreactivity.
The line produces normal amounts of p53 mRNA relative to normal lung.
For more information, please contact Cobioer (4008-750-250).

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