• 名称: NCI-H295R(人肾上腺皮质腺瘤)
  • 货号: CBP60794
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CBP60794
I. General information 
Synonyms: NCI-H295R  
Background: NCI-H295R was adapted from the NCI-H295 pluripotent adrenocortical carcinoma cell line (ATCC CRL-10296) established by A.F. Gazdar and associates from a carcinoma of the adrenal cortex. The original cells were adapted to a culture medium which decreased the population doubling time from 5 days to 2 days. While the original cells grew in suspension, the adapted cells were selected to grow in a monolayer. This cell line retains the ability to produce adrenal androgens. It is responsive to angiotensin II and potassium ions.
Species: Homo sapiens, human
Tissue: adrenal gland/cortex
Disease: Carcinoma
Gender: female,48 years,Black
Morphology: epithelial
Growth Mode: adherent
DNA Profile: Amelogenin: X
CSF1PO: 10,12
D13S317: 13
D16S539: 11
D5S818: 12
D7S820: 9,12
THO1: 9.3
TPOX: 8
vWA: 17,18
Cobioer’s Cell Line Authentication Service
Culture Medium: DMEM:F12 + 0.00625 mg/ml insulin+ 0.00625 mg/ml transferrin;+6.25 ng/ml selenium+1.25 mg/ml bovine serum albumin+ 0.00535 mg/ml linoleic acid; +2.5% Nu-Serum I. The additives
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Genes Expressed: aldosterone; cortisol; C19 steroids
Cellular Products: aldosterone; cortisol; C19 steroids
Comments: It is responsive to angiotensin II and potassium ions.
This cell line retains the ability to produce adrenal androgens.
For more information, please contact Cobioer (4008-750-250).

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