• 名称: UT-7(人类原巨核细胞白血病细胞)
  • 货号: CBP60809
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CBP60809
I. General information 
Synonyms: UT-7
Background: established from the bone marrow of a 64-year-old man with acute myeloid leukemia (AML M7) at diagnosis in 1988; cells are constitutively cytokine-dependent and responsive to various cytokines
Species: human (Homo sapiens)
Tissue: N/A 
Disease: acute myeloid leukemia
Gender: N/A 
Morphology: round (relatively large) cells growing singly in suspension (ca. 1-2% cells are slightly adherent); 
Growth Mode: N/A 
Doubling Time: ca. 70 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium: RPMI-1640+10%FBS+3IU/ml EPO
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD4 -, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 -, cyCD68 +;
Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile
Species: confirmed as human with IEF of LDH, NP
Cytogenetics: human flat-moded hypotetraploid karyotype - 72-88<4n>XXYY, +1, -8, -9, -9, -9, -11, -11, -12, -13, -13, -14, -18, -21, -22, +6mar, del(1)(q42)x2, t(2;4)(p16-23;q27-31)x1-2, der(2)t(2;5)(p16-23;q12-14)x1-2, add(5)(q11), del(5)(q13), der(6;13)(p10;q10), i(7q)x1-2, add(8)(q24)x1-3, del(9)(q22), add(12)(p12-13), der(13)t(13;13)(p11;q14), add(14)(p11), add(15)(p11), add(16)(p1?), add(17)(p11), add(19)(p13)x2, add(19)(q13), i(21q)x1-2 - extensive subclonal variation present - resembles published karyotype
Virus   ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).

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