• 名称: 647-V(人恶性2级膀胱癌细胞)
  • 货号: CBP60831
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CBP60831
I. General information 
Synonyms: 647-V
Background: established from a male patient with a primary urothelial bladder carcinoma (malignant, grade 2)
Species: human (Homo sapiens)
Tissue: bladder 
Disease: urothelial bladder carcinoma
Gender: N/A 
Morphology: adherent epithelial-like cells growing in monolayers
Growth Mode: N/A 
Doubling Time: ca. 30 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium: DMEM+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -
Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile
Species: confirmed as human with IEF of AST, NP
Cytogenetics: human flat-moded hyperdiploid/hypotriploid karyotype with 12% polyploidy - 52-60<3n>XX, -X/Y, -2, -4, -6, -9, -10, -12, -12, -14, -15, -16, -17, -18, +5mar, del(1)(p32), del(2)(q23)/add(2)(q2?), add(3)(q27), add(3)(q1?), i(5p), i(6p), i(7p)x2, add(8)(q24), del(11)(q22q24), del(13)(q?11q?14), der(19)t(12;19)(q13;q13), add(21)(p11) - i(5p) associated with bladder cancer
Virus   ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).

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