• 名称: MFE-280(人子宫内膜腺癌细胞)
  • 货号: CBP60839
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CBP60839
I. General information 
Synonyms: MFE-280
Background: established from tumor tissue from a 77-year-old woman with recurrence of endometrial carcinoma (adenomatous, partly papillary, grade G3) in 1990; described as forming heterotransplantable tumors in nude mice and as carrying receptors for progesterone
Species: human (Homo sapiens)
Tissue: endometrium  
Disease: endometrium adenocarcinoma
Gender: N/A 
Morphology: epitheloid cells growing adherently in monolayers 
Growth Mode: N/A 
Doubling Time: ca. 60-90 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium: MEM + 10% FBS+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP)
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin +
Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile
Species: confirmed as human with IEF of AST, LDH, NP
Cytogenetics: human hyperdiploid karyotype with 24% polyploidy - 52(48-52)<2n>X, -X, +1, +1, -2, +9, -10, -12, -13, -16, -17, -17, -18, -19, -20, -22, +18mar, add(1)(p34), add(1)(p3?), add(1)(q11), add(3)(q13), add(3)(q27-29), add(4)(p14), der(9)t(9;13)(p11;q11), add(15)(p11) - near diploidy and gain of 1q typical in endometrial carcinoma
Virus   ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).

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