• 名称: PNT1A(人正常成人前列腺上皮细胞(SV40))
  • 货号: CBP60848
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CBP60848
I. General information 
Synonyms: PNT1A
Background: Established by immortalisation of normal adult prostatic epithelial cells by transfection with a plasmid containing SV40 genome with a defective replication origin. The primary culture was obtained from the prostate of a 35 year old male at post mortem. The cells contain the SV40 genome and express large T protein. They present the phenotype of differentiated luminal prostatic cells with the expression of cytokeratin 8 and 18 (markers for luminal glandular epithelia) and vimentin. Cytokeratin14, a marker of epithelial basal cells, is not expressed. PNT1A cells are non-tumourigenic in nude mice.
Species: human (Homo sapiens)
Tissue: prostate
Disease: Human post pubertal prostate normal, immortalised with SV40
Gender: 35 year old male 
Morphology: Epithelial
Growth Mode: Adherent
Doubling Time: N/A
DNA Profile: Cobioer’s Cell Line Authentication Service
Culture Medium: RPMI 1640 + 10% FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Karyotype: Aneuploid
Subculture Routine: Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2;37°C.
Comments: For more information, please contact Cobioer (4008-750-250).

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