• 名称: MC3T3-E1 Subclone 14(小鼠颅顶前骨细胞亚克隆14)
  • 货号: CBP60868
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CBP60868
I. General information 
Synonyms: MC3T3-E1 Subclone 14
Background: A series of subclones were isolated from the cloned but phenotypically heterogeneous MC3T3-E1 cell line.
Species: Mus musculus, mouse
Tissue: bone, calvaria
Gender: newborn
Morphology: fibroblast
Growth Mode: adherent
DNA Profile: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12
D16S539: 9,11
D5S818: 12,13
D7S820: 8,12
THO1: 7
TPOX: 9,11
vWA: 17
Cobioer’s Cell Line Authentication Service
Culture Medium: Alpha-MEM(GIBCO#A1049001)+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Genes Expressed: collagen
Ref
Cellular Products: collagen
Ref
Tumor Formation: Yes, in immunodeficient mice
(forms bone-like ossicles)
Comments: The subclones were selected for high or low osteoblast differentiation and mineralization after growth in medium containing ascorbic acid.
The MC3T3-E1 Subclone 4 (ATCC CRL-2593) and the MC3T3 Subclone 14 (ATCC CRL-2594) lines exhibit high levels of osteoblast differentiation after growth in ascorbic acid and 3 to 4 mM inorganic phosphate.
They form a well mineralized extracellular matrix (ECM) after 10 days.
The MC3T3 Subclone 24 (ATCC CRL-2595) and the MC3T3 Subclone 30 (ATCC CRL-2596) lines exhibit poor osteoblast differentiation after growth in ascorbic acid. They do not form ECM. They can be used as negative controls for Subclones 4 and 14.Mineralizing subclones selectively express mRNAs for the osteoblast markers, bone sialoprotein (BSP), osteocalcin (OCN), and the parathyroid hormone (PTH)/parathyroid hormone-related protein (PTHrP) receptor.
Subclones with both high and low differentiation potential produce similar amounts of collagen in culture and express comparable basal levels of mRNA encoding Osf2/Cbfa1, an osteoblast-related transcription factor.
After implantation into immunodeficient mice, highly differentiating subclones form bone-like ossicles resembling woven bone, while poorly differentiating cells only produce fibrous tissue.
For more information, please contact Cobioer (4008-750-250)

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