• 名称: NE-4C (小鼠神经干细胞)
  • 货号: CBP60905
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CBP60905
I. General information 
Synonyms: NE-4C 
Background: The neuroepithelial cell lines, NE-4C (ATCC CRL-2925) and NE-GFP-4C (ATCC CRL-2926) were established from the cerebral vesicles of 9-day-old mouse embryos lacking the functional p53 genes. ­
Species: Mus musculus, mouse
Tissue: brain, neuroectodermal
Gender: embryo
Morphology: neuroepithelial
Growth Mode: adherent
Doubling Time: about 12 hours
Culture Medium: MEM+2mM L-Glutamine+10%FBS+1%NEAA+1%NaP
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Antigen Expression: Sox-2, Otx-2, En-1
Comments: The cells from both cell lines differentiated to neurons and astrocytes when exposed to retinoic acid. The GFP-transfected clone, NE-GFP-4C, when implanted into the forebrain of adult, new-born, and fetal mice or into the mid- and forebrain vesicles of early chick embryos was capable of developing morphologically differentiated neurons. Ref Ref
For more information, please contact Cobioer (4008-750-250).

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