• 名称: BV-2(C57BL/6小鼠脑小胶质细胞(永生化))
  • 货号: CBP60922
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CBP60922
I. General information 
Synonyms: BV-2
Background: This cell line was transformed by retroviruses
Species: Mus muscullus 
Tissue: Brain, microglial cells
Morphology: Loosely attached and in suspension
Subcultivation ratio: : 1:5 to 1:10
Medium: RPMI-1640+10%FBS
Cryopreservation medium: 90%FBS+10%DMSO
Comment The BV-2 cells express the nuclear v-myc and the cytoplasmic v-raf oncogene products as well as the env gp70 antigen at the surface level; the BV-2 cells have morphological, phenotypical and functional markers of macrophages
To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.

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