• 名称: MC3T3-E1(小鼠胚胎成颅骨成纤维细(可分化为成骨))
  • 货号: CBP60946
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CBP60946
I. General information 
Synonyms: MC3T3-E1
Background: established from the calvaria of an embryo/fetus C57BL/6 mouse; described in the literature to differentiate to osteoblasts and to produce collagen
Species: murine (mouse) (Mus musculus)
Tissue: embryo/fetus  
Disease: embryo/fetus calvaria fibroblasts
Gender: N/A 
Morphology: adherent fibroblast-like cells growing as monolayer
Growth Mode: N/A 
Doubling Time: ca. 1-2 days
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium: 80% MEM α, nucleosides (GIBCO 12571063) + 20% FBS + 2 mM L-glutamine+ 1 mM sodium pyruvate
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: N/A 
Fingerprint: N/A 
Species: originally confirmed as mouse with IEF of AST, MDH, NP and recently as mus musculus by COI DNA Barcoding
Cytogenetics: murine hypotetraploid karyotype with 11.5% polyploidy - 72(68-80)<4n> - centric fusion absent
Virus   ELISA: reverse transcriptase negative; PCR: SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).

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