• 名称: TM4(小鼠正常睾丸sertoli细胞)
  • 货号: CBP60969
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CBP60969
I. General information 
Synonyms: TM4
Species: Mus musculus, mouse
Tissue: testis
Gender: male, 11 to 13 days
Morphology: epithelial
Growth Mode: adherent
Culture Medium: DMEM:F12+5%HS+2.5%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Antigen Expression: H-Y antigen; Mus musculus, expressed
Receptor Expression: follicle stimulating hormone (FSH), expressed
androgen receptor, expressed
progesterone receptor, expressed
Genes Expressed: retinol binding protein, tissue plasminogen activator, transferrin
Tumor Formation: No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.
Comments: The TM4 cell line is reported to respond to FSH with an increase in cAMP production, but to not respond to luteinizing hormone (LH). The FSH responsiveness is much reduced compared to primary sertoli cell cultures. Constitutive plasminogen activator production is reported to be low, but is stimulated by FSH and, to a greater extent, by retinoic acid.
Tested and found negative for ectromelia virus (mousepox).
For more information, please contact Cobioer (4008-750-250).

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