• 名称: B82(小鼠皮肤成纤维细胞(对BrdU耐药))
  • 货号: CBP60977
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CBP60977
I. General information 
Synonyms: B82
Background: Derivative of NCTC clone 929 (ECACC catalogue no. 88102702) obtained by progressive selection in BUdR, has a cloning efficiency of 70-80%.
Species: Mouse  
Tissue: adipose
Disease: Mouse C3H/An sc areolar and adipose tissue
Gender: N/A
Morphology: Fibroblast
Growth Mode: Adherent
Doubling Time: N/A
DNA Profile: Cobioer’s Cell Line Authentication Service
Culture Medium: MEM+10%FBS+1%NEAA+1%NaP
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Karyotype: 2n = 51
Subculture Routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Comments: For more information, please contact Cobioer (4008-750-250).

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