• 名称: NK92(人恶性非霍奇金淋巴瘤患者的自然杀伤细胞)
  • 货号: CBP60980
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CBP60980
I. General information 
Synonyms: NK92
Background: NK-92 is an interleukin-2 (IL-2) dependent Natural Killer Cell line derived from peripheral blood mononuclear cells from a 50 year old Caucasian male with rapidly progressive non-Hodgkin's lymphoma.
Species: Homo sapiens, human
Tissue: peripheral blood
Disease: malignant non-Hodgkin's lymphoma
Gender: male,50 years,Caucasian
Morphology: lymphoblast
Growth Mode: suspension, multicell aggregates
DNA Profile: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 9,12
D16S539: 11,12
D5S818: 12,13
D7S820: 10,11
THO1: 6,9.3
TPOX: 8
vWA: 18
Cobioer’s Cell Line Authentication Service
Culture Medium: Alpha MEM without ribonucleosides and
deoxyribonucleosides+2 mM Lglutamine+1.5 g/L sodium bicarbonate+0.2 mM inositol+0.1 mM 2-mercaptoethanol+0.02 mM folic acid+100-200
U/ml recombinant IL2+12.5% horse serum+12.5%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Antigen Expression: CD2 +, CD7 +, CD11a +, CD28 + , CD45 +, CD54 +, CD56 +, CD1 -, CD3 -, CD4 -, CD5 -, CD8 -, CD10 -, CD14 -, CD16 -, CD19 -, CD20 -, CD23 -, CD34 -, HLA-DR -
Comments: The cell line is dependent on the presence of recombinant Il-2 and a dose as low as 10 U/mL is sufficient to maintain proliferation; cells will die within 72 hours in the absence of IL-2.
NK-92 cells have the following characteristics: surface marker positive for CD2, CD7, CD11a, CD28, CD45, CD54 and CD56 bright; surface marker negative for CD1, CD3, CD4, CD5, CD8, CD10, CD14, CD16, CD19, CD20, CD23, CD34 and HLA-DR.
ATCC confirmed this cell line is positive for the presence of Epstein-Barr viral DNA sequences via PCR.
For more information, please contact Cobioer (4008-750-250).

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