• 名称: 32D(小鼠IL-3依赖性细胞)
  • 货号: CBP60995
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CBP60995
I. General information 
Synonyms: 32D
Background: established from long-term bone marrow cultures of C3H/HeJ mice infected with the Friend murine leukemia virus; cells are constitutively growth factor-dependent
Species: murine (mouse) (Mus musculus)
Tissue: bone 
Disease: bone marrow
Gender: N/A 
Morphology: mostly round cells growing singly or in small clusters in suspension, some cells are loosely adherent;
Growth Mode: N/A 
Doubling Time: ca. 15 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium: RPMI1640+10%FBS+10U/ml rmIL-3
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD11b +, F4/80 (+); 
Fingerprint: N/A 
Species: originally confirmed as mouse with IEF of LDH, NP and with species PCR and recently as mus musculus by COI DNA Barcoding
Cytogenetics: murine hypodiploid karyotype - 35(34-35)<2n> - carries 5 centric fusion markers
Virus   ELISA: reverse transcriptase negative; PCR: SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).

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