• 名称: Ishikawa亚洲人子宫内膜腺癌
  • 货号: CBP61085
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CBP61085
I. General information 
Synonyms: Ishikawa
Background: The cell line Ishikawa was established from an endometrial adenocarcinoma from a 39 year old woman. The cells induced well differentiated adenocarcinoma in athymic nude mice. Estrogen and progesterone receptors were demonstrated both in cell culture and in induced tumours. Ishikawa has been reported to produce corticotropin-releasing hormone, placental alkaline phosphatase and chorionic gonadotrophin, and responded to steroid hormones. The cell line has been eradicated from mycoplasma at ECACC using BM-cyclin.
Ishikawa was temporarily removed from the web site whilst an authentication issue was investigated. Following collaboration with key investigators in this field, ECACC is confident that this cell line is Ishikawa.
Species: human (Homo sapiens)
Tissue: Uterus
Disease: Human Asian endometrial adenocarcinoma
Gender: 39 year old female
Morphology: Epithelial-like
Growth Mode: Adherent
Doubling Time: N/A
DNA Profile: Amelogenin: X
CSF1PO: 11,12
D13S317: 9,12
D16S539: 9
D5S818: 10,11
D7S820: 9,10
THO1: 9,10
TPOX: 8
vWA: 14,17
Cobioer’s Cell Line Authentication Service
Culture Medium: MEM+1% NEAA+5% FBS+1%NaP
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Karyotype: Not specified
Subculture Routine: Split sub-confluent cultures (70-80%) 1:5  i.e. seeding at 2-4x10,000 cells/cm²  using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37ºC; cells tend to pile up.
Comments: For more information, please contact Cobioer (4008-750-250).

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