• 名称: NK92MI(人恶性非霍奇金淋巴瘤患者的自然杀伤细胞)
  • 货号: CBP61113
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CBP61113
I. General information 
Synonyms: NK92MI
Background: NK-92MI is an interleukin-2 (IL-2) independent Natural Killer Cell line derived from the NK-92 (ATCC CRL-2407) cell line by transfection. NK-92 is an interleukin-2 (IL-2) dependent Natural Killer Cell line derived from peripheral blood mononuclear cells from a 50 year old Caucasian male with rapidly progressive non-Hodgkin's lymphoma. The parental cells were transfected with human IL-2 cDNA in the retroviral MFG-hIL-2 vector by particle-mediated gene transfer. The transfection is stable.
Species: Homo sapiens, human
Tissue: peripheral blood, blood
Disease: malignant non-Hodgkin's lymphoma
Gender: male,50 years, Caucasian
Morphology: lymphoblast
Growth Mode: suspension, multicell aggregates
Doubling Time: N/A
DNA Profile: D5S818: 12. 13
D13S317: 9, 12
D7S820: 10, 11
D16S539: 11, 12
vWA: 16, 18
THO1: 6, 9.3
TPOX: 8
CSF1PO: 11, 12
Amelogenin: X, Y
Cobioer’s Cell Line Authentication Service
Culture Medium: Alpha MEM without ribonucleosides and deoxyribonucleosides+2 mM L-glutamine+1.5 g/L sodium bicarbonate+ 0.2 mM inositol+ 0.1 mM 2-mercaptoethanol+ 0.02 mM folic acid+ 12.5% HS+ 12.5% FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Antigen Expression: N/A
Receptor Expression: N/A
Oncogene: N/A
Genes Expressed: N/A
Cellular Products: N/A
Tumor Formation: N/A
Comments:  
NK-92 and this derivative cell line NK-92MI have the following characteristics: surface marker positive for CD2, CD7, CD11a, CD28, CD45, CD54 and CD56 bright; surface marker negative for CD1, CD3, CD4, CD5, CD8, CD10, CD14, CD16, CD19, CD20, CD23, CD34 and HLA-DR.
The parental IL-2 dependent cell line is available as ATCC CRL-2407 (NK-92). NK-92MI was shown to contain, express, and synthesize the hIL-2.
A culture submitted to the ATCC in September of 1998 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative.
ATCC confirmed this cell line is positive for the presence of Epstein-Barr viral DNA sequences via PCR.
For more information, please contact Cobioer (4008-750-250).

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