• 名称: COLO-320(人结肠腺癌细胞)
  • 货号: CBP61130
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CBP61130
I. General information 
Synonyms: COLO-320
Background: established from the tumor mass of a 55-year-old Caucasian woman with a moderately undifferentiated adenocarcinoma of the sigmoid colon in 1977; cells were described in the literature to produce several hormones, e.g. serotonin, epinephrine; cells express MYC mRNA
Species: human (Homo sapiens)
Tissue: colon 
Disease: colon adenocarcinoma
Gender: N/A 
Morphology: undifferentiated, round cells in suspension and loosely adherent in monolayers; 
Growth Mode: N/A 
Doubling Time: ca. 38 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium: RPMI-1640+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin +
Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile
Species: confirmed as human with IEF of AST, G6PD, MDH, NP
Cytogenetics: human hyperdiploid karyotype - 53(50-54)<2n>XX, -3, -3, +5, +7, +12, -13, -17, +18, +22, +6mar, t(1;5) (p11;q11), der(5)t(5;?)(p15;?), der(7)t(7;?)(q22;?), der(9)t(9;?)(p11;?), del(9)(p22), del(12)(p12), i(13q), der(14)t(14;?)(q24;?), der(18)t(18;?)(p11;?) - variable numbers of supernumerary dmins were present in 90% of cells
Virus   ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).

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