• 名称: DR4 MEF小鼠胚胎成纤维细胞
  • 货号: CBP61134
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CBP61134
I. General information 
Synonyms: DR4 MEF
Background: The cell line was established by ATCC in 2003 from embryonic day 14 (E14) DR4 mouse embryos obtained from The Jackson Laboratory (Stock# 003208).  Mouse embryo fibroblasts (MEFs) prepared from the DR4 mouse strain are resistant to neomycin, hygromycin, puromycin, and 6-thioguanine.
Dr. Rudolf Jaenisch at the Massachusetts Institute of Technology developed the DR4 mouse strain by the intercrossing of three different strains: one bearing resistance genes neoR and puroR, a second bearing the resistance gene hygR, and a third bearing a natural deletion encompassing the Hprt gene. A series of matings incorporated all 4 drug resistance genes into the strain (Tucker et al., 1997; PubMed: 9278500). The background of the original DR4 strain was a mix of 129S4/SvJae, 129P2/OlaHsd, BALB/c, and C57BL/6.
Species: Mus musculus, mouse
Tissue: Embryo
Disease: malignant histiocytosis
Gender: Male and female mixed, 14 days gestation embryo
Morphology: fibroblast
Growth Mode: adherent
Doubling Time: N/A 
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium: DMEM+15%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Antigen Expression: N/A 
Receptor Expression: N/A 
Oncogene: N/A 
Genes Expressed: N/A 
Cellular Products: N/A 
Tumor Formation: N/A 
Comments: The growth of these cells should be arrested before being used as a feeder layer. ATCC has successfully irradiated and treated the cells with Mitomycin C for use as a feeder layer. If the MEFs are being used as a feeder layer for ES cells, it is not recommended to use them past passage no. 7 (P7).
ATCC tested that this cell line is resistant to:
G 418 (neomycin): 200 microgm/mL
Puromycin: 0.4 microgm/mL
Hygromycin: 110 microgm/mL
6-Thioguanine: 2.5 microgm/mL
For more information, please contact Cobioer (4008-750-250).

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