• 名称: C75人Dukes'D期的中度分化的人结直肠腺癌
  • 货号: CBP61183
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CBP61183
I. General information 
Synonyms: C75
Background: The C75 cell line was established from a 56-year old male patient with a moderately well differentiated adenocarcnoma of the sigmoid colon classified as Dukes' stage D
Species: human (Homo sapiens)
Tissue: Colon
Disease: Human colorectal adenocarcinoma, moderately well differentiated, Dukes' stage D
Gender: a 56-year old male 
Morphology: Epithelial
Growth Mode: Adherent
Doubling Time: N/A
DNA Profile: Amelogenin: X,Y
CSF1PO: 12
D13S317: 10,13
D16S539: 8,11
D5S818: 12
D7S820: 9,11
THO1: 8,9.3
TPOX: 10
vWA: 17
Cobioer’s Cell Line Authentication Service
Culture Medium: IMDM + 10% FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Karyotype: N/A
Subculture Routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C.
C75 cells grow very slowly (see growth curve); following resuscitation or subculture the cells take at least 48 hours to re-attach. Cells should be left without disturbance during this time to facilitate adhesion. Centrifugation of the cells (100g x 5 min) at resuscitation to remove DMSO improves the establishment of a viable culture. Once attached, the cells grow in discrete islands and use of trypsin or trypsin/EDTA to subculture the cells (even without knocking the flask) yields large clumps. Further disaggregation may be achieved by repeatedly pipetting the cells.
Comments: For more information, please contact Cobioer (4008-750-250).

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