• 名称: VM-CUB-1人膀胱移行细胞癌细胞
  • 货号: CBP61187
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CBP61187
I. General information 
Synonyms: VM-CUB-1
Background: established from the primary transitional cell carcinoma of the urinary bladder of a male patient; described in the literature to carry a p53 point mutation
Species: human (Homo sapiens)
Tissue: urinary bladder 
Disease: urinary bladder transitional cell carcinoma
Gender: N/A 
Morphology: adherent epithelial-like cells growing as monolayers;
Growth Mode: N/A 
Doubling Time: ca. 30-45 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium: DMEM+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -
Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile
Species: confirmed as human with IEF of AST, NP
Cytogenetics: human hypertriploid karyotype with 4% polyploidy - 80<3n>XX/XX?Y, +1, +1, +5, +6, +7, +9, -11, +15, -18, +19, +20, +22, +22, +7mar, del(1)(q21), del(1)(q11), der(1;6)(p10;q10)x2, add(3)(p21.3)x2, i(5p), der(6)t(1;6)(q11;q22), ?inv(7)(p15q35), i(9q), del(10)(p11), del(10)(q?22?24), add(12)(p11)x2, add(15)(p11), add(21)(p11) - sideline with i(5q)
Virus   ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).

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