• 名称: TSA201(稳定表达SV40温度敏感性T抗原的转化的人胚肾细胞)
  • 货号: CBP61213
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CBP61213
I. General information 
Synonyms: TSA201
Background: tsA201 is a transformed human kidney (293, ECACC catalogue no. 85120602) cell line stably expressing an SV40 temperature-sensitive T antigen. The cell line has been used in a variety of functional expression assays and has been reported to produce high levels of recombinant proteins. Transfection efficiency decreases over a period of several months, therefore it is advisable to lay down a frozen stock as a reserve. The cells have been eradicated from mycoplasma at ECACC. The identity of tsA201 and 293 has been confirmed by STR profiling.
Species: human (Homo sapiens)
Tissue: Kidney
Disease: Human embryonal kidney, SV40 transformed
Gender: N/A
Morphology: Epithelial-like
Growth Mode: Adherent
Doubling Time: N/A
DNA Profile: Amelogenin: X
CSF1PO: 11,12
D13S317: 12
D16S539: 9,13
D5S818: 8,9
D7S820: 11
THO1: 7,9.3
TPOX: 11
vWA: 16,19
Cobioer’s Cell Line Authentication Service
Culture Medium: DMEM+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Karyotype: Not specified
Subculture Routine: Split sub-confluent cultures (70-80%) 1:2 to 1:8;i.e. seeding at 2-4x10,000 cells/cm² using ; mechanical agitation or EDTA solution; 5% CO2; 37°C. Do not let cells become over confluent as this can affect morphology.
Comments: For more information, please contact Cobioer (4008-750-250).

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