• 名称: EGFR D770_N771insNPG/BaF3
  • 货号: CBP73180
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CBP73180
I. Introduction

Cell Line Name:

EGFR D770_N771insNPG/BaF3

Gene Synonyms:

ERBB, mENA, ERBB1, HER1 

Host Cell:

Ba/F3

Stability: 16 passages

Application:

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Freeze Medium:

90% FBS+10% DMSO

Complete Culture Medium:

1640+10%FBS+2ug/ml puromycin

Mycoplasma Status:

Negative

 
II.Background

EGFR is the receptor for members of the EGF family and is a transmembrane glycoprotein that has tyrosine kinase activity. Binding of epidermal growth factor to EGFR induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation, differentiation, motility, and cell survival (1). Activation of EGFR triggers mitogenic signaling in gastrointestinal mucosa, and its expression is upregulated in colon cancers and most neoplasms (2). Activation of EGFR triggers activation of the ERK-signaling pathway in normal gastric epithelial and colon cancer cell lines. Inactivation of EGFR with selective inhibitors significantly reduces ERK2 activation, c-fos mRNA expression and cell proliferation.

 
III. Representative Data

1. Anti-proliferation assay

 

 

Figure . Anti-proliferation assay of three reference compounds on the EGFR D770_N771insNPG/BaF3 Stable Cell Line

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