• 名称: CD40/NFkB-Luc/HEK293
  • 货号: CBP74009
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CBP74009
I. Background
CD40, a TNF receptor superfamily member, was initially identified on B lymphocytes. However, antigen presenting cells (APCs) such as monocytes, basophils, dendritic cells and non-immune cells like endothelial cells and epithelial cells have been found to express CD40. A wide variety of carcinoma cells also over-express CD40. Interaction with CD40 ligand (CD40L, CD154) on CD4+ T helper lymphocytes triggers the expression of intercellular adhesion molecule (ICAM) and other pro-inflammatory cytokines. CD40:CD40L signaling simultaneously increases activation of antigen-specific T cells. CD40 also activates NF-κB-dependent signaling in response to lipopolysaccharide (LPS) found on Gram negative bacterial pathogens. Furthermore, agonistic CD40 monoclonal antibodies have been shown to activate antigen presenting cells (APC) and promote anti-tumor T-cell responses in addition to fostering cytotoxic myeloid cells with the potential to control cancer in the absence of T-cell immunity.
 
II. Description

Recombinant HEK293 cell line expressing full length human CD40 (Tumor necrosis factor receptor superfamily member 5; TNFRSF5). Expression is confirmed by real-time qPCR and western blot. This NF-ĸB luciferase reporter construct is stably integrated into the genome. The firefly luciferase gene is controlled by 4 copies of NF-kB response element located upstream of the TATA promoter. Following activation by human CD40 ligand, NF-ĸB transcription factors bind to the DNA response elements to induce transcription of the luciferase gene.

 
III. Introduction
Host Cell: HEK293
Expressed gene: CD40
Stability: 32 passages
Synonym(s): NF-κB cell line, NFkappaB cell line, luciferase reporter, NF-kB cell line, NFkB cell line, CD40 cell line, cd40 luciferase reporter, CD40 HEK293 cell line
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: MEM + 10% FBS+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP)+1ug/ml puromycin+100ug/ml hygromycin
Mycoplasma Testing: Negative
Storage: Liquid nitrogen
Application(s): The CD40 NF-ĸB Reporter HEK293 Stable Cell Line is ideal for High Throughput Screening (HTS) to identify potential CD40 agonistic monoclonal antibodies and CD40-specific inhibitors.
 
IV. Description of Host Cell Line
Organism: Human
Tissue: kidney
Morphology: Epitheloid cell
Growth Properties: Adherent
 
Ⅴ. Representative Data

 

Figure 1. Recombinant HEK293 cell line expressing firefly luciferase gene under the control of NFκB response elements with constitutive expression of CD40.

Figure 2. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. HEK293-CD40-NFκB Luciferase Reporter cells were stimulated by CD40L, the EC50 was 43ng/ml.

 

 

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