• 名称: CSF1R/SRE-Luc/HEK293
  • 货号: CBP74010
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CBP74010
I. Background

Colony Stimulating Factor 1 Receptor (CSF1R, CD115, M-CSF-R) is the receptor for the cytokine CSF1 (MCSF; CSF-1), which controls the growth, function, and differentiation of macrophages. This interaction drives the development and survival of Tumor-Associated Macrophages (TAMs), which in turn suppress the local immune response to the cancer. An alternative ligand is IL-34, which has been implicated in neuroprotective and survival signals following brain injury and neurodegeneration.

As a tyrosine kinase transmembrane receptor, activation of CSF1R by its ligand initiates a vast array of intracellular activity, including activation of the MAPK/ERK signaling pathway. When phosphorylated by ERK, Elk1 forms a complex with Serum Response Factor (SRF) and binds to Serum Response Element (SRE), resulting in the expression of numerous mitogen-inducible genes.

 
Ⅱ. Introduction
Host Cell: HEK293
Expressed gene:

CFS1R-SRE-Luciferase

Stability: 32 passages
Synonym(s): FMS, CD115
Freeze Medium: 90% FBS+10% DMSO
Culture Medium:

DMEM +10%FBS+200ug/ml hygromycin+1ug/ml puromycin

Mycoplasma Testing: Negative
Storage: Liquid nitrogen
Application(s): • Screen for activators or inhibitors of the CSF1R/MAPK/ERK signaling pathway.
 
Ⅲ. Description of Host Cell Line
Organism: Human
Tissue: Embryonic kidney
Morphology: Epitheloid cell
Growth Properties: Adherent
 
Ⅳ. Representative Data

Figure 1. Recombinant HEK293 cell line expressing firefly luciferase gene under the control of CRE response elements with constitutive expression of CSF1R(Colony Stimulating Factor 1 Receptor)

Figure 2. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. HEK293/CFS1R-SRE Luciferase Reporter cells were stimulated by CSF1, the EC50 was 3.75ng/ml.

 

 Figure 3. HEK293/CFS1R-SRE Luciferase Reporter cells were stimulated by CSF1 inhibitors.

 

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