• 名称: FOXP3 promoter-Luc/Jurkat
  • 货号: CBP74011
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CBP74011
I. Background
Foxp3, belonging to the forkhead family, is a master transcription factor that expresses exclusively in regulatory T cells, a subset of CD4+ T cells. Regulation of Foxp3 is critical for maintaining immunological tolerance. Over-expression of Foxp3 is known to suppress effector T cell activation.
 
II. Description

Human Foxp3 luciferase reporter construct is stably integrated into the genome of Jurkat T- cells. The firefly luciferase gene is controlled by a human Foxp3 promoter and an enhancer-like conserved noncoding sequence upstream of the Foxp3 promoter. 

Foxp3 Reporter (Luc) - Jurkat Recombinant Cell Line

Figure 1. Illustration of Foxp3 promoter region with representative transcription factor binding sites and enhancer regions.

 
III. Introduction
Host Cell: Jurkat
Expressed gene: Foxp3-Luciferase
Stability: 32 passages
Synonym(s): Foxp3, Foxp3 cell line, scurfin cell line, foxo
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: RPMI-1640+10%FBS+1 ug/ml puromucin
Storage: Liquid nitrogen
Application(s): The Foxp3 promoter Jurkat reporter cell line is suitable for monitoring the transcription activity of Foxp3 in response to stimulant, and establishing cell-based screens for inhibitors that target specific Foxp3-stimulating molecules.
 
IV. Description of Host Cell Line
Organism: Homo sapiens, human
Tissue: Peripheral blood
Disease: Acute T cell leukemia
Morphology: Lymphoblast
Growth Properties: Suspension
 
Ⅴ. Representative Data

Figure 1. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. Jurkat/Foxp3 Luciferase Reporter cells were stimulated by Lonomycin, the EC50 was 0.21 ng/ml.

 

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