• 名称: IL2_promoter-Luc/Jurkat
  • 货号: CBP74014
  • 浏览次数: 609
  • 已销售数量:0件
  • 评论信息
  • 购买数量:  购买数量不能低于最少订购数量!

购买 询价 收藏 QQ交流 好友推荐

 

CBP74014
I. Background
The particles contain a firefly luciferase gene driven by the human IL-2 promoter (Figure 1). After transduction, activation of the IL-2 signaling pathway in the target cells can be monitored by measuring the luciferase activity.
 
II. Description
The IL-2 Promoter Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells.
 
III. Introduction
Host Cell: Jurkat
Expressed gene: IL-2-Luciferase
Stability: 32 passages
Synonym(s): IL-2 Promoter reporter, lentivirus reporter, HIV reporter, VSV-G reporter
Freeze Medium: 90% FBS+10% DMSO
Culture Medium:  
Mycoplasma Testing: Negative
Storage: Liquid nitrogen
Application(s): • Screen for activators or inhibitors of IL-2 signaling pathway in transduced target cells
• Generation of IL-2 promoter luciferase reporter stable cell line
 
IV. Description of Host Cell Line
Organism: Homo sapiens, human
Tissue: Peripheral blood
Disease: Acute T cell leukemia
Morphology: Lymphoblast
Growth Properties: Suspension
 
Ⅴ. Representative Data

 

 Figure 1. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System.

 

Figure 2. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. Jurkat-IL-2 Luciferase Reporter cells were stimulated by anti-CD3, the EC50 was 8.31ng/ml.

 

关键词: