• 名称: OX40/NFkB-Luc/HEK293
  • 货号: CBP74017
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CBP74017
I. Background
Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40, is a member of the TNFR-superfamily of receptors. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation. This receptor has been shown to activate NF-kappaB through its interaction with adaptor proteins TRAF2 and TRAF5. Its ligand, OX40L, binds to OX40 receptors on T-cells, preventing them from dying and subsequently increasing cytokine production. OX40 has a critical role in the maintenance of an immune response beyond the first few days and onwards to a memory response due to its ability to enhance survival.
 
II. Description
Recombinant HEK293 cell expressing firefly luciferase gene under the control of NF-κB response elements with constitutive expression of human OX40 (Tumor Necrosis Factor Receptor Superfamily Member 4, TNFRSF4, and CD134, GenBank Accession No. NM_003327)
 
III. Introduction
Host Cell: HEK293
Expressed gene: NF-κB-Luciferase-OX40
Stability: 32 passages
Synonym(s): OX40, OX40 cell Line, CD134 Cell Line, ox40 nfkb
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: MEM + 10% Foetal Bovine Serum (FBS)+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) +1ug/ml puromycin+100ug/ml hygromycin
Mycoplasma Testing: Negative
Storage: Liquid nitrogen
Application(s): Screen for activators or inhibitors of OX40 signaling in a cellular context and characterize the biological activity of OX40 and its interactions with ligands
 
IV. Description of Host Cell Line
Organism: Homo sapiens, human
Tissue: Embryonic kidney
Disease: Normal
Morphology: Epitheloid cell
Growth Properties: Adherent
 
Ⅴ. Representative Data

Figure 1. Recombinant HEK293 cell expressing firefly luciferase gene under the control of NF-κB response elements with constitutive expression of human OX40 (Tumor Necrosis Factor Receptor Superfamily Member 4, TNFRSF4, and CD134, GenBank Accession No. NM_003327)

 

Figure 2. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. HEK293/NF-κB Luciferase-OX40 Reporter cells were stimulated by OX40L, the EC50 was 82.7 ng/ml.


 

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