• 名称: TLR8/NFkB-Luc/HEK293
  • 货号: CBP74021
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CBP74021
I. Background
The TLR family plays a fundamental role in pathogen recognition and activation of innate immunity. TLR8 is an endosomal receptor that recognizes single stranded RNA (ssRNA), and can recognize ssRNA viruses such as Influenza, HIV and HCV. TLR8 binding to the viral RNA recruits MyD88 and leads to activation of the transcription factor NF-κB and an antiviral response. Human TLR8 is functional but murine TLR8 is non-functional because it lacks five amino acids.
 
II. Description

Recombinant HEK293 cell expressing firefly luciferase gene under the control of NFkB response elements with constitutive expression of human TLR8 (Toll-like receptor 8, also known as CD288, a member of the toll-like receptor (TLR) family (GenBank Accession No. NM_138636).

 
III. Introduction
Host Cell: NFAT reporter Jurkat cells
Expressed gene: TLR8
Stability: 32 passages
Synonym(s): CD288, TLR8, Toll-like receptor 8, tlr, tlr-8 TLR, nfkb, nf-kb reporter TLR
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: MEM + 10% Foetal Bovine Serum (FBS)+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) +1ug/ml puromycin+100ug/ml hygromycin
Mycoplasma Testing: Negative
Storage: Liquid nitrogen
Application(s): Screen for activators or inhibitors of TLR8 signaling in a cellular context • Characterize the biological activity of TLR8 and its interactions with ligands
 
IV. Description of Host Cell Line
Organism: Human
Tissue: kidney
Morphology: Epitheloid cell
Growth Properties: Adherent
 
Ⅴ. Representative Data

 

 

Figure 1. Recombinant HEK293 cell line expressing firefly luciferase gene under the control of NFκB response elements with constitutive expression of TLR8.

 

 

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