• 名称: OE19(人食管癌细胞)
  • 货号: CBP60495
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CBP60495
I. General information 
Synonyms: OE19
Background: The cell line OE19, also known as JROECL19, was established in 1993 from an adenocarcinoma of gastric cardia/oesophageal gastric junction of a 72 year old male patient. The tumour was identified as pathological stage III (UICC) and showed moderate differentiation. The cell line OE19 expresses HLA-A, -B and -C antigens (MHC class I) constitutively. Treatment with interferon-gamma induced the expression of ICAM-1 (CD54). Expression of HLA-DR (MHC class II) on interferon-gamma addition was only measured in a sub-population of OE19. The cells express epithelial cytokeratins and are tumourigenic in nude mice.The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
Species: human (Homo sapiens)
Tissue: Oesophagus/gastric cardia
Disease: Human Caucasian oesophageal carcinoma
Gender: N/A
Morphology: Epithelial
Growth Mode: Adherent
Doubling Time: N/A
DNA Profile: Amelogenin: X
CSF1PO: 11,13
D13S317: 9,11
D16S539: 12,13
D5S818: 11,14
D7S820: 8
THO1: 8,9
TPOX: 8
vWA: 16,17
Cobioer’s Cell Line Authentication Service
Culture Medium: RPMI1640+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Karyotype: Aneuploid
Subculture Routine: Split sub-confluent cultures (70-80%) 1:8 i.e. seeding at 2-4 x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.These cells grow slowly in islands and when resuscitated from frozen it can take up to 7 days to reach 70% confluence.
Comments: For more information, please contact Cobioer (4008-750-250).

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