• 名称: OE33(人食管癌细胞)
  • 货号: CBP60496
  • 浏览次数: 87
  • 已销售数量:0件
  • 评论信息
  • 购买数量:  购买数量不能低于最少订购数量!

购买 询价 收藏 QQ交流 好友推荐

CBP60496
I. General information 
Synonyms: OE33
Background: The cell line OE33, also known as JROECL33, was established from the adenocarcinoma of the lower oesophagus (Barrett's metaplasia) of a 73 year old female patient. The tumour was identified as pathological stage IIA (UICC) and showed poor differentiation. OE33 express HLA-A, -B and -C antigens (MHC class I) and ICAM-1 constitutively. Expression of HLA-DR (MHC class II) can be induced by treatment with interferon-gamma. The cells express epithelial cytokeratins and are tumourigenic in nude mice.
Cultures derived from ECACC stocks of this cell line have been whole genome sequenced (Contino et al 2016) confirming the presence of many of the known mutations that drive oesophageal cancer
Species: human (Homo sapiens)
Tissue: oesophagus
Disease: 73 year old female
Gender: Human Caucasian oesophageal carcinoma
Morphology: Epithelial
Growth Mode: Adherent
Doubling Time: N/A
DNA Profile: Amelogenin: X
CSF1PO: 10
D13S317: 14
D16S539: 12
D5S818: 11
D7S820: 9,10
THO1: 7,8
TPOX: 8,11
vWA: 17
Cobioer’s Cell Line Authentication Service
Culture Medium: RPMI1640+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Karyotype: Aneuploid
Subculture Routine: Split sub-confluent cultures (70-80%) 1:8 i.e. seeding at 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Initially these cells grow slowly and can take up to 7 days until ready for the next split, 50% media changes will be necessary every 2-3 days (i.e. replacing half the old medium with fresh).
Comments: For more information, please contact Cobioer (4008-750-250).
Cell Line Overview Mutation Copy Number Variation
Gene Expression RNAi(siRNA/shRNA/sgRNA...) Compound Sensitivity Profile

科佰生物免费提供各类科研数据,请联系我们4008-750-250索取,暂时仅对会员开放。

如下仅展示案例细胞A549数据:

 

Cell Line Overview: Download

Synonyms:

A 549; A549; NCI-A549; A549/ATCC; A549 ATCC; hA549

Background:

This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old Caucasian male. Cells were described to induce tumors in athmyic mice and to synthesize lecithin.

Species:

Homo sapiens, human

Tissue:

Lung

Disease:

Carcinoma

Gender:

Male, 58 years adult, Caucasian

Morphology:

Epithelial cells, growing adherently as monolayer

Growth Mode:

Adherent

Doubling Time:

24~40hrs

DNA Profile:

Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 11
D7S820: 8,11
THO1: 8,9.3
TPOX: 8,11

vWA: 14
Cobioer’s Cell Line Authentication Service

Culture Medium:

F-12K+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium:

90% Complete Medium+10%DMSO

Photo:

 

 

Mutation: Download
Gene Name(s) Gene Transcript AA Syntax CDS Syntax Mutation Type
AATK ENSG00000181409 ENST00000326724 p.E1219Q c.3655G>C Substitution - Missense
ABCA9 ENSG00000154258 ENST00000340001 p.M1I c.3G>T Substitution - Missense
ABCB1 ENSG00000085563 ENST00000265724 p.Y1267C c.3800A>G Substitution - Missense
ABI3BP ENSG00000154175 ENST00000471714 p.R104Q c.311G>A Substitution - Missense
ABI3BP ENSG00000154175 ENST00000284322 p.R104Q c.311G>A Substitution - Missense
ADH1A ENSG00000187758 ENST00000209668 p.I209V c.625A>G Substitution - Missense
AGTR1 ENSG00000144891 ENST00000349243 p.H132N c.394C>A Substitution - Missense

 

Copy Number Variation: Downlonad
Ensembl Gene Gene Name(s) Copy Number Signal Chromosome
ENSG00000237269 RBMY2TP 0 loss Y
ENSG00000227949 CYCSP46 0 loss Y
ENSG00000234803 FAM197Y2 0 loss Y
ENSG00000230066 FAM197Y3 0 loss Y
ENSG00000237802 FAM197Y6 0 loss Y
ENSG00000228383 FAM197Y7 0 loss Y
ENSG00000233699 TTTY18 0 loss Y
ENSG00000238073 RBMY2HP 0 loss Y

 

Gene Expression: Download
Gene Relative level of mRNA Gene Relative level of mRNA
NOC2L -1.66 SKI -0.75
KLHL17 0.15 MORN1 -0.43
PLEKHN1 0.51 RER1 -0.76
C1orf170 1.12 PEX10 -0.43
HES4 -0.22 PLCH2 0.34
ISG15 -3.09 PANK4 -1.64
AGRN 2.48 HES5 -1.87
RNF223 -1.74 TNFRSF14 -1.37

 

RNAi(siRNA/shRNA/sgRNA...):
Design according to the gene, please contact us 4008-750-250.

 

Compound Sensitivity Profile:Download
Drug Name  Targets  Z Score
Tanespimycin HSP90 -2.656303
Selumetinib MEK1, MEK2 -2.057352
NU7441 DNAPK -1.983103
KU-55933 ATM -1.962478
Refametinib MEK1, MEK2 -1.742238
TW 37 BCL2, BCL-XL, MCL1 -1.739538
AZD8055 MTORC1, MTORC2 -1.667451

关键词: