• 名称: COLO-699(人肺癌腺癌细胞)
  • 货号: CBP60157
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CBP60157
I. General information 
Synonyms: COLO-699
Background: established from the pleural fluid of a 57-year-old woman with adenocarcinoma of the lung in 1986
Species: human (Homo sapiens)
Tissue: lung  
Disease: lung adenocarcinoma
Gender: N/A 
Morphology: adherent, epitheloid cells growing as monolayers
Growth Mode: N/A 
Doubling Time: ca. 38 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium: RPMI-1640+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin +; cells are HMB-45 -positive (this marker is highly associated with melanomas - hence the cells may be melanoma-derived)
Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile
Species: confirmed as human with IEF of AST, MDH
Cytogenetics: human hypertriploid karyotype with 2% polyploidy - 70(62-72)<3n>XX, -X, +1, +3, -4, +6, +7, +7, +8, -9, -10, +12, -14, -16, -17, -17, +18, +20, der(2)t(2;10)(q27;q12), der(7)t(7;?)(p21;?)x2, i(10q), der(11)t(8;11)(q12;q14), idic(16q), der(21)t(21;?)(p12;?)x2 - a supernumerary small ring chromosome was present in 28% cells - corresponds to original karyotype with reinterpretation
Virus   ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).

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