• 名称: NCI-H69(人小细胞肺癌细胞)
  • 货号: CBP60180
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CBP60180
I. General information 
Synonyms: NCI-H69
Species: Homo sapiens, human
Tissue: lung
Disease: carcinoma; small cell lung cancer
Gender: male, 55 years adult, Caucasian
Morphology: floating aggregates
Growth Mode: suspension, multicell aggregates
DNA Profile: CSF1PO: 10, 12
D13S317: 12
D16S539: 11
D5S818: 11, 13
D7S820: 9
THO1: 8, 9
TPOX: 10
vWA: 16, 17
Amelogenin: XY
Cobioer’s Cell Line Authentication Service
Culture Medium: RPMI-1640+20%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Receptor Expression: insulin-like growth factor II (IGF II)
Oncogene: myc +; myb +; fes +; fms +; raf +; ras +
Genes Expressed: Oncogenes: myc +; myb +; fes +; fms +; raf +; ras +
Tumor Formation: Yes, in nude mice
(The cells form tumors with typical small cell carcinoma histology.)                        Yes,  forms colonies in soft agar
Comments: This cell line is aneuploid, will form colonies in soft agar and retains small cell carcinoma morphology and ultrastructure as well as APUD cell characteristics.
The cells grow in aggregates, thus cell counts are not accurate.
The cells stain positively for cytokeratins.
The line can be adapted to grow in shaker flask or spinner flask systems.
The N-myc gene is amplified, and there is expression of the mRNA and protein.
C-myc mRNA, but not protein, is expressed at a low level.
There is expression of c-myb, v-fes, v-fms, c-raf 1, Ha-ras, Ki-ras and N-ras mRNA.
For more information, please contact Cobioer (4008-750-250).

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