• 名称: HL-60(人白血病肿瘤细胞)
  • 货号: CBP60517
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CBP60517
I. General information 
Synonyms: HL-60
Background: HL-60 is a promyelocytic cell line derived by S.J. Collins, et al. Peripheral blood leukocytes were obtained by leukopheresis from a 36-year-old Caucasian female with acute promyelocytic leukemia.
Species: Homo sapiens, human
Tissue: peripheral blood
Disease: acute monocytic leukemia
Gender: Caucasian,female, 36 years 
Morphology: myeloblastic
Growth Mode: suspension
DNA Profile: D5S818: 12
D13S317: 8,11
D7S820: 11,12
D16S539: 11
vWA: 16
THO1: 7,8
Amelogenin: X
TPOX: 8,11
CSF1PO: 13,14
Cobioer’s Cell Line Authentication Service
Culture Medium: RPMI-1640+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Receptor Expression: complement, expressed,Ref     Fc, expressed ,Ref
Oncogene: myc +
Genes Expressed: tumor necrosis factor (TNF), also known as tumor necrosis factor alpha (TNF-alpha, TNF alpha), after stimulation with phorbol myristic acid,myc +
Cellular Products: tumor necrosis factor (TNF), also known as tumor necrosis factor alpha (TNF-alpha, TNF alpha), after stimulation with phorbol myristic acid
Tumor Formation: Yes, in nude mice (subcutaneous myeloid tumors),Yes, in semi-solid media
Comments: HL-60 cells spontaneously differentiate and differentiation can be stimulated by butyrate, hypoxanthine, phorbol myristic acid (PMA, TPA), dimethylsulfoxide (DMSO, 1% to 1.5%), actinomycin D, and retinoic acid.
The cells exhibit phagocytic activity and responsiveness to chemotactic stimuli. The line is positive for myc oncogene expression.
For more information, please contact Cobioer (4008-750-250).

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