• 名称: SV40LT-SMC Clone HEP-SA (大鼠平滑肌细胞)
  • 货号: CBP60611
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CBP60611
I. General information 
Synonyms: SV40LT-SMC Clone HEP-SA 
Background: This is a clone of the SV40LT-SMC cell line derived from primary abdominal aortic tissue from young rats. The parental line was created by immortalizing the primary cells by transfection with SV40 large T antigen.
Species: Rattus norvegicus, rat
Tissue: aorta; smooth muscle
Gender: 3 months, rat
Morphology: fibroblast
Growth Mode: adherent
DNA Profile: Cobioer’s Cell Line Authentication Service
Culture Medium: DMEM+10%NBCS+200 mcg/mL G418
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Genes Expressed: alpha actin; large T antigen
Cellular Products: alpha actin; large T antigen
Comments: This is a clone of the SV40LT-SMC cell line derived from primary abdominal aortic tissue from young rats.  The parental line was created by immortalizing the primary cells by transfection with SV40 large T antigen. Transfection was done using conditioned medium from psiTEX cells (which produce a helper free replication defective retrovirus that carries the sequences for large T antigen and neomycin (G418) resistance).
Clone HEP-SA cells maintain smooth muscle cell characteristics including smooth cell alpha actin expression and growth inhibition by heparin. Large T antigen is detectable in the nuclei.
For more information, please contact Cobioer (4008-750-250).

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