• 名称: MonoMac6(人急性单核细胞白血病细胞)
  • 货号: CBP60521
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CBP60521
I. General information 
Synonyms: MONO-MAC-6
Background: established from the peripheral blood of a 64-year-old man with relapsed acute monocytic leukemia (AML FAB M5) in 1985 following myeloid metaplasia; carries t(9;11)(p22;q23) leading to KMT2A-MLLT3 (MLL-MLLT3; MLL-AF9) fusion gene; more information on this cell line on the following website: "www.monocytes.de"; sister cell line of MONO-MAC-1 (DSM ACC 252); cells are used for the detection of pyrogens
Species: human (Homo sapiens)
Tissue: N/A 
Disease: acute monocytic leukemia
Gender: N/A 
Morphology: single, round/multiformed cells or small clusters of cells in suspension, sometimes loosely adherent; 1-5% giant cells
Growth Mode: N/A 
Doubling Time: ca. 50-60 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium: RPMI-1640+10%FBS+0.1mM NEAA+ 1 mM sodium pyruvate + 10 µg/ml insulin
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD4 -, CD13 +, CD14 +, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +; note that CD14 expression depends on cultivation conditions; CD14 positivity cannot be guaranteed; image
Fingerprint: same DNA profile as MONO-MAC-1 using multiplex PCR of minisatellite markers
Species: confirmed as human with IEF of AST, MDH, NP
Cytogenetics: human flat-moded hypotetraploid karyotype with near-diploid (8%) and polyploid (17%) sidelines - 84-90<4n>XX/XXX, -Y, +6, +7, -12, -13, -13, -16, -16, +2mar, t(9;11)(p22;q23)x2, add(10)(p11)x2, add(12)(q21), del(13)(q13q14)der(13)t(13;14)(p11;q12)x2, der(17)t(13;17)(q21;p11)x2 - first cell line shown to carry t(9;11) characteristic of AML M5
Molec. Genetics: expression of fusion gene KMT2A-MLLT3 (MLL-MLLT3; MLL-AF9) confirmed by RT-PCR
Virus   ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).

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