• 名称: PC-3(人前列腺癌细胞)
  • 货号: CBP60343
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CBP60343
I. General information 
Synonyms: PC-3
Background: Established from a grade 4 prostatic adenocarcinoma from a 62 year old male Caucasian. The cells grow in agar and produce tumours in nude mice. Exhibit low acid phosphatase and testosterone-5-alpha reductase activity. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
Species: human (Homo sapiens)
Tissue: prostate
Disease: Human Caucasian prostate adenocarcinoma
Gender: 62 year old ,male,  Caucasian
Morphology: Epithelial
Growth Mode: Adherent
Doubling Time: N/A
DNA Profile: Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 11
D5S818: 13
D7S820: 8,11
THO1: 6,7
TPOX: 8,9
vWA: 17
Cobioer’s Cell Line Authentication Service
Culture Medium: F12K+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Karyotype: 2n = 46, triploid, modal no. 62
Subculture Routine: Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C. Medium change every 5 days. The initial subculture interval after cells are thawed may be longer than 7-9 days.
Comments: For more information, please contact Cobioer (4008-750-250).

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