• 名称: HMT-3522 S1(人类乳腺上皮细胞)
  • 货号: CBP60351
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CBP60351
I. General information 
Synonyms: HRA-19
Background: HMT-3522 S1, also known as HMT-3522/wt, is a subline that has been derived from HMT-3522. The parent line was established from a benign breast tumour of a 48 year old woman and has undergone spontaneous malignant transformation. It was grown on collagen in serum-free media without antibiotics from explantation onwards. A mutation at codon 179 of the p53 gene was detected during establishment of the parent line.
HMT-3522 S1 is a continuation of this line from passage 34 and is grown without collagen.
It is near-diploid, but an evolution of karyotype has been observed by regular chromosomal analyses. The expression of cytokeratin 13, 14, 17 and 18 and vimentin has been reported. Expression of EGF-R, c-erb-B2, N-myc, c-ras-Ha1, c-ras-Ki2 and N-ras was shown to fluctuate slightly with passage number. The cells have tested negative for estrogen receptor, c-erbA1, c-erb-A2, int-2 or hst-1. During propagation growth factor requirements decreased simultaneously with amplification and overexpression of c-myc protooncogene. In a three-dimensional basement membrane culture assay the phenotypic characteristics of normal breast tissue in vivo are recapitulated. This cell line has remained non-tumorigenic for more than 400 passages. Publications using this cell line must refer to the original paper (Briand et al., In Vitro Cell Dev Biol 1987; 23:181). Any sublines created must include the name HMT-3522 in the designation, but a short name may be used in the same paper.
Subline HMT-3522 S2 (ECACC catalogue no. 98102211) has been isolated by culturing S1 cells from passage 118 in serum-free media without epidermal growth factor (EGF). This cell line grows on collagen-treated flasks.
The third subline, HMT-3522 T4-2 (ECACC catalogue no. 98102212) was established from S2 at passage 238 and is the only tumorigenic line in nude mice out of the 3 sublines. This cell line grows on collagen-treated flasks.
Species: human (Homo sapiens)
Tissue: Breast
Disease: Human Caucasian breast epithelial
Gender: N/A
Morphology: Epithelial-like
Growth Mode: Adherent
Doubling Time: N/A
DNA Profile: Amelogenin: X
CSF1PO: 11
D13S317: 9,13
D16S539: 14
D5S818: 11,12
D7S820: 11,13
THO1: 8,9.3
TPOX: 8,9
vWA: 14,17
Cobioer’s Cell Line Authentication Service
Culture Medium: DMEM / Ham's F12 (1:1) + 2mM Glutamine + 250ng/ml insulin + 10µg/ml transferrin +10E-8M sodium selenite + 10E-10M 17 beta-estradiol + 0.5µg/ml hydrocortisone + 5µg/ml ovine prolactin + 10ng/ml EGF
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Karyotype: 45,XX,1p-,-6,8q+-,12p+,14q+,17q+
Subculture Routine: If starting from a frozen ampoule the cryoprotectant should be removed. Add thawed cells to a conical based centrifuge tube e.g. 15ml tube. Slowly add 4 ml of culture medium and re-suspend cells, taking a sample of the cell suspension, e.g. 100μl, to count. Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes.
Split sub-confluent cultures using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells are slow to detach from the flask. Add the same volume of trypsin inhibitor as trypsin used, centrifuge and reseed cells at 2-4 x10, 000 cells/cm². Medium is serum-free, therefore trypsin inhibitor is essential.
S1cells can be slow in spreading out after subculture and may take 2-3 days to attach. Good growth occurs 5-7 days after subculture.
Cells should be frozen in (45% conditioned media: 45% fresh media) with 10% DMSO.
Comments: For more information, please contact Cobioer (4008-750-250).
Cell Line Overview Mutation Copy Number Variation
Gene Expression RNAi(siRNA/shRNA/sgRNA...) Compound Sensitivity Profile

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如下仅展示案例细胞A549数据:

 

Cell Line Overview: Download

Synonyms:

A 549; A549; NCI-A549; A549/ATCC; A549 ATCC; hA549

Background:

This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old Caucasian male. Cells were described to induce tumors in athmyic mice and to synthesize lecithin.

Species:

Homo sapiens, human

Tissue:

Lung

Disease:

Carcinoma

Gender:

Male, 58 years adult, Caucasian

Morphology:

Epithelial cells, growing adherently as monolayer

Growth Mode:

Adherent

Doubling Time:

24~40hrs

DNA Profile:

Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 11
D7S820: 8,11
THO1: 8,9.3
TPOX: 8,11

vWA: 14
Cobioer’s Cell Line Authentication Service

Culture Medium:

F-12K+10%FBS
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium:

90% Complete Medium+10%DMSO

Photo:

 

 

Mutation: Download
Gene Name(s) Gene Transcript AA Syntax CDS Syntax Mutation Type
AATK ENSG00000181409 ENST00000326724 p.E1219Q c.3655G>C Substitution - Missense
ABCA9 ENSG00000154258 ENST00000340001 p.M1I c.3G>T Substitution - Missense
ABCB1 ENSG00000085563 ENST00000265724 p.Y1267C c.3800A>G Substitution - Missense
ABI3BP ENSG00000154175 ENST00000471714 p.R104Q c.311G>A Substitution - Missense
ABI3BP ENSG00000154175 ENST00000284322 p.R104Q c.311G>A Substitution - Missense
ADH1A ENSG00000187758 ENST00000209668 p.I209V c.625A>G Substitution - Missense
AGTR1 ENSG00000144891 ENST00000349243 p.H132N c.394C>A Substitution - Missense

 

Copy Number Variation: Downlonad
Ensembl Gene Gene Name(s) Copy Number Signal Chromosome
ENSG00000237269 RBMY2TP 0 loss Y
ENSG00000227949 CYCSP46 0 loss Y
ENSG00000234803 FAM197Y2 0 loss Y
ENSG00000230066 FAM197Y3 0 loss Y
ENSG00000237802 FAM197Y6 0 loss Y
ENSG00000228383 FAM197Y7 0 loss Y
ENSG00000233699 TTTY18 0 loss Y
ENSG00000238073 RBMY2HP 0 loss Y

 

Gene Expression: Download
Gene Relative level of mRNA Gene Relative level of mRNA
NOC2L -1.66 SKI -0.75
KLHL17 0.15 MORN1 -0.43
PLEKHN1 0.51 RER1 -0.76
C1orf170 1.12 PEX10 -0.43
HES4 -0.22 PLCH2 0.34
ISG15 -3.09 PANK4 -1.64
AGRN 2.48 HES5 -1.87
RNF223 -1.74 TNFRSF14 -1.37

 

RNAi(siRNA/shRNA/sgRNA...):
Design according to the gene, please contact us 4008-750-250.

 

Compound Sensitivity Profile:Download
Drug Name  Targets  Z Score
Tanespimycin HSP90 -2.656303
Selumetinib MEK1, MEK2 -2.057352
NU7441 DNAPK -1.983103
KU-55933 ATM -1.962478
Refametinib MEK1, MEK2 -1.742238
TW 37 BCL2, BCL-XL, MCL1 -1.739538
AZD8055 MTORC1, MTORC2 -1.667451

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